view article
Figure 4
Validation of the reconstructions from the unsupervised iterative 2D classification workflow. (a) Resolution of ab initio reconstructions across three different CryoSift scores using the FSC0.5 against simulated volumes. (b) Resolution improvement from CryoSift score 4.5 to 3.5, showing the removal of unsuitable particles. (c) All-atom inclusion into ab initio reconstructions across three different CryoSift scores using the mean all-atom Q-score. (d) All-atom inclusion improvement from CryoSift score 4.5 to 3.5. (e) Ab initio reconstructions used for (a)–(d) color-coded by the CryoSift scores. Ab initio reconstructions were calculated without imposing symmetry (C1) and color-coded by their respective CryoSift score, 2.5 (coral), 3.5 (blue) and 4.5 (tan), and ordered by increasing molecular weight. Failed reconstructions for aldolase and PRex-1G are highlighted in red.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 81| Part 12| December 2025| Pages 517-526
https://doi.org/10.1107/S2053230X25008866
Follow Acta Cryst. F
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS