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Figure 5
ResLog analysis of the CryoSift thresholds. Validation of the reconstruction quality using the ResLog plot (inverse resolution over the logarithm of the number of particles). The slope of the linear regression line, given as the B factor in Å2, is an indicator of the data quality (with lower meaning worse quality). Plots were calculated using cryoSPARC's ResLog analysis at FSC 0.134. Half-set splits were generated from ab initio reconstructions using the `Homogeneous reconstruction only' job to preserve the initial angular assignments.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 81| Part 12| December 2025| Pages 517-526
https://doi.org/10.1107/S2053230X25008866
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