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Figure 2
Gel-filtration chromatography profiles of two MBP-fused Mb-P′ constructs. (a) Gel-filtration profile of Mb-P′ expressed without an MBP fusion. (b) Gel-filtration profiles of MBP–Mb-P′ fusion constructs carrying different MBP–monobody linker sequences: NAAATGS (black) and TSGTGS (gray). In both panels, inverted triangles indicate the elution positions of molecular-mass standards. Arrows indicate the elution peaks corresponding to the Mb-P′ protein in (a) and the MBP–Mb-P′ fusion proteins in (b).

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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