 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 3]](bus5006fig3mag.jpg)
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Figure 3
Generation of a ΔcybC strain for bacterial BAG2 expression. (a) Gel electrophoresis of colony PCR amplifying the WT T7 Express cybC locus using flanking primers. (b) Gel electrophoresis of colony PCR amplifying the T7 Express ΔcybC::aph locus using flanking primers. (c) SDS–PAGE of Protein L elution fractions following BAG2 overexpression in T7 Express ΔcybC indicating no co-purifying proteins. (d) Cation-exchange trace of pooled Protein L elution fractions following BAG2 overexpression in T7 Express ΔcybC. (e) SDS–PAGE of cation-exchange fractions following BAG2 overexpression in T7 Express ΔcybC. (f) Size-exclusion chromatography trace following incubation of a BRIL fusion protein (∼37 kDa) with BAG2 purified from T7 Express ΔcybC indicating complete formation of the desired complex. (g) SDS–PAGE of the size-exclusion chromatography fractions following incubation of a BRIL fusion protein (∼37 kDa) with BAG2 purified from T7 Express ΔcybC. |
![[Figure 3]](bus5006fig3.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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