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Figure 3
Performance of amyloid processing using cryoSPARC. (a) Map–model fits and xy slices with scale bars for refined reconstructions of amyloid datasets from EMPIAR. Light-chain amyloids (AL; PDB entry 9oka), β2-microglobulin variant V27M (b2m; PDB entry 8a7q), lysozyme variant D87G (Lys; PDB entry 8r4a), ex vivo PHF-type tau (PDB entry 6hre), transthyretin amyloids (ATTR; PDB entry 8ade) and recombinant tau MIA-type amyloids (PDB entry 7qkl). Single-protofilament polymorphs are shown in blue and multi-protofilament polymorphs in blue and orange. (b) Cardiac ATTR reconstructions from our previous work showing two wild-type transthyretin samples and familial variant V122I (indicated by an arrow). (c) Validation of re-processed EMPIAR datasets (orange) compared with the original depositions (blue, from Table 1[link]) with resolutions estimated according to an FSC 0.143 cutoff, Q-scores were calculated with ChimeraX and helical symmetry parameters.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 82| Part 7| July 2026|
https://doi.org/10.1107/S2053230X26003675
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