most read articles

These are the most read articles in the last six months.

Crystal structures of glutamyl-tRNA synthetase from Elizabethkingia anopheles and E. meningosepticum. L. Brooks, S. Subramanian, D. M. Dranow, S. J. Mayclin, P. J. Myler & O. A. Asojo (2022). Acta Cryst. F78, 306-312.

Relating protein crystal structure to ligand-binding thermodynamics. J. R. Helliwell (2022). Acta Cryst. F78, 403-407.

Serial crystallography with multi-stage merging of thousands of images. A. S. Soares, Y. Yamada, J. Jakoncic, S. McSweeney, R. M. Sweet, J. Skinner, J. Foadi, M. R. Fuchs, D. K. Schneider, W. Shi, B. Andi, L. C. Andrews & H. J. Bernstein (2022). Acta Cryst. F78, 281-288.

In situ crystal data-collection and ligand-screening system at SPring-8. H. Okumura, N. Sakai, H. Murakami, N. Mizuno, Y. Nakamura, G. Ueno, T. Masunaga, T. Kawamura, S. Baba, K. Hasegawa, M. Yamamoto & T. Kumasaka (2022). Acta Cryst. F78, 241-251.

Variations on a theme: crystal forms of the amino-acid transporter MhsT. C. Neumann, D. Focht, S. Trampari, J. A. Lyons & P. Nissen (2022). Acta Cryst. F78, 297-305.

Delineating the activity of the potent nicotinic acetylcholine receptor agonists (+)-anatoxin-a and (−)-hosieine-A. H. P. Parker, A. Dawson, M. J. Jones, R. Yan, J. Ouyang, R. Hong & W. N. Hunter (2022). Acta Cryst. F78, 313-323.

Erwinia tasmaniensis levansucrase shows enantiomer selection for (S)-1,2,4-butanetriol. I. Polsinelli, M. Salomone-Stagni & S. Benini (2022). Acta Cryst. F78, 289-296.

6-Phosphogluconate dehydrogenase and its crystal structures. S. Hanau & J. R. Helliwell (2022). Acta Cryst. F78, 96-112.

Crystal structure of the kringle domain of human receptor tyrosine kinase-like orphan receptor 1 (hROR1). S. R. Guarino, A. Di Bello, M. Palamini, M. C. Capillo & F. Forneris (2022). Acta Cryst. F78, 185-192.

Crystal structure of an inorganic pyrophosphatase from Chlamydia trachomatis D/UW-3/Cx. J. Maddy, B. L. Staker, S. Subramanian, J. Abendroth, T. E. Edwards, P. J. Myler, K. Hybiske & O. A. Asojo (2022). Acta Cryst. F78, 135-142.

Conformational flexibility in the zinc solute-binding protein ZnuA. E. L. Yekwa, F. A. Serrano & E. Yukl (2022). Acta Cryst. F78, 128-134.

A crystal-processing machine using a deep-ultraviolet laser: application to long-wavelength native SAD experiments. Y. Kawano, M. Hikita, N. Matsugaki, M. Yamamoto & T. Senda (2022). Acta Cryst. F78, 88-95.

Crystal structure of a hypothetical protein from Giardia lamblia. D. K. Beard, S. Bristol, K. Cosby, A. Davis, C. Manning, L. Perry, L. Snapp, A. Toy, K. Wheeler, J. Young, B. Staker, T. L. Arakaki, J. Abendroth, S. Subrahamanian, T. E. Edwards, P. J. Myler & O. A. Asojo (2022). Acta Cryst. F78, 59-65.

Expression, purification and crystallization of a novel metagenome-derived salicylaldehyde dehydrogenase from Alpine soil. S. U. Dandare, M. Håkansson, L. A. Svensson, D. J. Timson & C. C. R. Allen (2022). Acta Cryst. F78, 161-169.

Crystal structures of the ligand-binding domain of human peroxisome proliferator-activated receptor δ in complexes with phenylpropanoic acid derivatives and a pyridine carboxylic acid derivative. T. Oyama, K. Takiguchi & H. Miyachi (2022). Acta Cryst. F78, 81-87.

Crystal structure of an extracellular superoxide dismutase from Onchocerca volvulus and implications for parasite-specific drug development. A. Moustafa, M. Perbandt, E. Liebau, C. Betzel & S. Falke (2022). Acta Cryst. F78, 232-240.

Crystal structure of the ternary complex of Leishmania major pteridine reductase 1 with the cofactor NADP⁺/NADPH and the substrate folic acid. L. Dello Iacono, F. Di Pisa & S. Mangani (2022). Acta Cryst. F78, 170-176.

Biochemical and crystallization analysis of the CENP-SX–DNA complex. S. Ito & T. Nishino (2022). Acta Cryst. F78, 193-199.

Crystal structure of betaine aldehyde dehydrogenase from Burkholderia pseudomallei. D. K. Beard, S. Subramanian, J. Abendroth, D. M. Dranow, T. E. Edwards, P. J. Myler & O. A. Asojo (2022). Acta Cryst. F78, 45-51.

Crystal structure of a hypothetical protein from Giardia lamblia. Corrigendum. D. K. Beard, S. Bristol, K. Cosby, A. Davis, C. Manning, L. Perry, L. Snapp, A. Toy, K. Wheeler, J. Young, B. Staker, T. L. Arakaki, J. Abendroth, S. Subramanian, T. E. Edwards, P. J. Myler & O. A. Asojo (2022). Acta Cryst. F78, 143.

Structure of the Monkeypox virus profilin-like protein A42R reveals potential functional differences from cellular profilins. G. Minasov, N. L. Inniss, L. Shuvalova, W. F. Anderson & K. J. F. Satchell (2022). Acta Cryst. F78, 371-377.

Crystal structure of a short-chain dehydrogenase/reductase from Burkholderia phymatum in complex with NAD. J. Alenazi, S. Mayclin, S. Subramanian, P. J. Myler & O. A. Asojo (2022). Acta Cryst. F78, 52-58.

The structure of Synechococcus elongatus enolase reveals key aspects of phosphoenolpyruvate binding. J. M. González, R. Martí-Arbona, J. C.-H. Chen & C. J. Unkefer (2022). Acta Cryst. F78, 177-184.

Structure of angiogenin dimer bound to double-stranded RNA. K. Sievers & R. Ficner (2022). Acta Cryst. F78, 330-337.

Crystal structure of the Rho-associated coiled-coil kinase 2 inhibitor belumosudil bound to CK2α. P. Brear & M. Hyvönen (2022). Acta Cryst. F78, 348-353.

Room-temperature serial synchrotron crystallography of the human phosphatase PTP1B. S. Sharma, A. Ebrahim & D. A. Keedy (2023). Acta Cryst. F79, 23-30.

Crystallization and preliminary X-ray analysis of the C-type lectin domain of the spicule matrix protein SM50 from Strongylocentrotus purpuratus. Corrigendum. R. Ebenhoch, P. Juneja, A. Rao, H. Cölfen, K. Diederichs & W. Welte (2022). Acta Cryst. F78, 347.

Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature. T. Tandrup, L. Lo Leggio & F. Meilleur (2023). Acta Cryst. F79, 1-7.

The structure of a tautomerase superfamily member linked to the type VI secretion system of Acinetobacter baumannii. G. Pankov, G. Mol Avelar, G. Buchanan, S. J. Coulthurst & W. N. Hunter (2023). Acta Cryst. F79, 8-16.

The structure of His-tagged Geobacillus stearothermophilus purine nucleoside phosphorylase reveals a `spanner in the works'. F. M. Given, F. Moran, A. S. Johns, J. A. Titterington, T. M. Allison, D. L. Crittenden & J. M. Johnston (2022). Acta Cryst. F78, 416-422.

Crystals of SctV from different species reveal variable symmetry for the cytosolic domain of the type III secretion system export gate. D. Gilzer, E. Baum, N. Lieske, J. L. Kowal & H. H. Niemann (2022). Acta Cryst. F78, 386-394.

Structure of an H3N2 influenza virus nucleoprotein. M. L. Knight, H. Fan, D. L. V. Bauer, J. M. Grimes, E. Fodor & J. R. Keown (2021). Acta Cryst. F77, 208-214.

Crystal structure of the TLDc domain of human NCOA7-AS. M. Arnaud-Arnould, M. Tauziet, O. Moncorgé, C. Goujon & M. Blaise (2021). Acta Cryst. F77, 230-237.

The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina. A. J. Kosgei, M. D. Miller, M. Bhardwaj, W. Xu, J. S. Thorson, S. G. Van Lanen & G. N. Phillips Jr (2022). Acta Cryst. F78, 1-7.

Cryotrapping peroxide in the active site of human mitochondrial manganese superoxide dismutase crystals for neutron diffraction. J. Azadmanesh, W. E. Lutz, L. Coates, K. L. Weiss & G. E. O. Borgstahl (2022). Acta Cryst. F78, 8-16.

The crystal structures of the enzyme hydroxymethylbilane synthase, also known as porphobilinogen deaminase. J. R. Helliwell (2021). Acta Cryst. F77, 388-398.

Crystal structures of FolM alternative dihydrofolate reductase 1 from Brucella suis and Brucella canis. I. Porter, T. Neal, Z. Walker, D. Hayes, K. Fowler, N. Billups, A. Rhoades, C. Smith, K. Smith, B. L. Staker, D. M. Dranow, S. J. Mayclin, S. Subramanian, T. E. Edwards, P. J. Myler & O. A. Asojo (2022). Acta Cryst. F78, 31-38.

Crystal structures of HER3 extracellular domain 4 in complex with the designed ankyrin-repeat protein D5. F. Radom, C. Vonrhein, P. R. E. Mittl & A. Plückthun (2021). Acta Cryst. F77, 192-201.

Structures of the Plasmodium falciparum heat-shock protein 70-x ATPase domain in complex with chemical fragments identify conserved and unique binding sites. N. Mohamad, A. O'Donoghue, A. L. Kantsadi & I. Vakonakis (2021). Acta Cryst. F77, 262-268.

Crystal structure of a putative short-chain dehydrogenase/reductase from Paraburkholderia xenovorans. J. Davidson, K. Nicholas, J. Young, D. G. Conrady, S. Mayclin, S. Subramanian, B. L. Staker, P. J. Myler & O. A. Asojo (2022). Acta Cryst. F78, 25-30.

Structural characterization of DynU16, a START/Bet v1-like protein involved in dynemicin biosynthesis. S. K. Alvarado, M. D. Miller, M. Bhardwaj, J. S. Thorson, S. G. Van Lanen & G. N. Phillips Jr (2021). Acta Cryst. F77, 328-333.

Crystal structure of human CRM1, covalently modified by 2-mercaptoethanol on Cys528, in complex with RanGTP. A. Shaikhqasem, K. Schmitt, O. Valerius & R. Ficner (2021). Acta Cryst. F77, 70-78.

The identification and structural analysis of potential 14-3-3 interaction sites on the bone regulator protein Schnurri-3. L. Soini, S. Leysen, T. Crabbe, J. Davis & C. Ottmann (2021). Acta Cryst. F77, 254-261.

Ibuprofen: a weak inhibitor of carbonic anhydrase II. J. Combs, J. Andring & R. McKenna (2022). Acta Cryst. F78, 395-402.

Roles of the hydroxy group of tyrosine in crystal structures of Sulfurisphaera tokodaii O⁶-methylguanine-DNA methyltransferase. M. Kikuchi, T. Yamauchi, Y. Iizuka & M. Tsunoda (2021). Acta Cryst. F77, 444-451.

Crystallographic analysis and phasing of iron-assimilating protein 1 (FEA1) from Chlamydomonas reinhardtii. L. Juniar, V. Adlfar, M. Hippler, H. Tanaka & G. Kurisu (2021). Acta Cryst. F77, 134-139.

Engineering and crystal structure of a monomeric FLT3 ligand variant. E. Pannecoucke, L. Raes & S. N. Savvides (2021). Acta Cryst. F77, 121-127.

Phosphorus and sulfur SAD phasing of the nucleic acid-bound DNA-binding domain of interferon regulatory factor 4. A. Agnarelli, K. El Omari, R. Duman, A. Wagner & E. J. Mancini (2021). Acta Cryst. F77, 202-207.

Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB. C. Geerds, A. Haas & H. H. Niemann (2021). Acta Cryst. F77, 246-253.

A new crystal form of GABARAPL2. K. Scicluna, G. Dewson, P. E. Czabotar & R. W. Birkinshaw (2021). Acta Cryst. F77, 140-147.

Crystal structure of the [2Fe–2S] protein I (Shethna protein I) from Azotobacter vinelandii. B. V. Kabasakal, C. A. R. Cotton & J. W. Murray (2021). Acta Cryst. F77, 407-411.

Finding order in chaos – nanocrystals in amorphous protein gels. M. W. Bowler (2021). Acta Cryst. F77, 386-387.

Topical Reviews in Acta Crystallographica F Structural Biology Communications. J. R. Helliwell, J. Newman & M. J. van Raaij (2021). Acta Cryst. F77, 385.

Submission of structural biology data for review purposes. E. N. Baker, C. S. Bond, E. F. Garman, J. Newman, R. J. Read & M. J. van Raaij (2021). Acta Cryst. F77, 435-436.

Biochemical and biophysical characterization of the OXA-48-like carbapenemase OXA-436. B. A. Lund, A. M. Thomassen, T. J. W. Carlsen & H.-K. S. Leiros (2021). Acta Cryst. F77, 312-318.

Crystal structures of Val58Ile tryptophan repressor in a domain-swapped array in the presence and absence of L-tryptophan. J. Sprenger, C. L. Lawson, C. von Wachenfeldt, L. Lo Leggio & J. Carey (2021). Acta Cryst. F77, 215-225.

Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site. C. D. Radka & S. G. Aller (2021). Acta Cryst. F77, 286-293.

Purification, crystallization and crystallographic analysis of the PorX response regulator associated with the type IX secretion system. A. Saran, N. Weerasinghe, C. J. Thibodeaux & N. Zeytuni (2022). Acta Cryst. F78, 354-362.

The structure of succinyl-CoA synthetase bound to the succinyl-phosphate intermediate clarifies the catalytic mechanism of ATP-citrate lyase. J. Huang & M. E. Fraser (2022). Acta Cryst. F78, 363-370.

Crystal structure of the capsular polysaccharide-synthesis enzyme CapG from Staphylococcus aureus. N. Tien, C.-Y. Ho, S.-J. Lai, Y.-C. Lin, C.-S. Yang, Y.-C. Wang, W.-C. Huang, Y. Chen & J.-J. Chang (2022). Acta Cryst. F78, 378-385.

Crystal structure of the C-terminal domain of the plant-specific microtubule-associated protein Spiral2. M. Ohno, Y. Higuchi & I. Hayashi (2023). Acta Cryst. F79, 17-22.

Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase. T. Murakawa, M. Suzuki, T. Arima, M. Sugahara, T. Tanaka, R. Tanaka, S. Iwata, E. Nango, K. Tono, H. Hayashi, K. Fukui, T. Yano, K. Tanizawa & T. Okajima (2021). Acta Cryst. F77, 356-363.

Crystallization and low-resolution structure solution of the SALM3–PTPσ synaptic adhesion complex. S. Karki & T. Kajander (2022). Acta Cryst. F78, 39-44.

Structure of the hypothetical protein TTHA1873 from Thermus thermophilus. I. Yuvaraj, S. K. Chaudhary, J. Jeyakanthan & K. Sekar (2022). Acta Cryst. F78, 338-346.

X-ray structure of a human cardiac muscle troponin C/troponin I chimera in two crystal forms. C. Yan & J. S. Sack (2022). Acta Cryst. F78, 17-24.

Crystallization and crystallographic studies of a novel chickpea 11S globulin. L. Sun, A. Zhou & F. Zhang (2022). Acta Cryst. F78, 324-329.

Crystal structure of Sphingobacterium multivorum serine palmitoyltransferase complexed with tris(hydroxymethyl)aminomethane. H. Ikushiro, A. Takahashi, T. Murakami, A. Katayama, T. Sawai, H. Goto, I. Miyahara, N. Kamiya & T. Yano (2022). Acta Cryst. F78, 408-415.

Identification, structure determination and analysis of Mycobacterium smegmatis acyl-carrier protein synthase (AcpS) crystallized serendipitously. I. Bhatia, S. Yadav & B. K. Biswal (2022). Acta Cryst. F78, 252-264.

Crystal structure of hemoglobin from mouse (Mus musculus) compared with those from other small animals and humans. S. S. Sundaresan, P. Ramesh, N. Shobana, T. Vinuchakkaravarthy, S. Yasien & M. N. G. Ponnuswamy (2021). Acta Cryst. F77, 113-120.

Structure of mitogen-activated protein kinase kinase 1 in the DFG-out conformation. S. Nakae, M. Kitamura, D. Fujiwara, M. Sawa, T. Shirai, I. Fujii & T. Tada (2021). Acta Cryst. F77, 459-464.

Crystal structure determination of the armadillo repeat domain of Drosophila SARM1 using MIRAS phasing. W. Gu, Z. Luo, C. Vonrhein, X. Jia, T. Ve, J. D. Nanson & B. Kobe (2021). Acta Cryst. F77, 364-373.

Crystal structure of the catalytic domain of human RPTPH. M. Kim & S. E. Ryu (2022). Acta Cryst. F78, 265-269.

Crystal structure of thermally stable homodimeric cytochrome c′-β from Thermus thermophilus. T. Yoshimi, S. Fujii, H. Oki, T. Igawa, H. R. Adams, K. Ueda, K. Kawahara, T. Ohkubo, M. A. Hough & Y. Sambongi (2022). Acta Cryst. F78, 217-225.

Nanobody-aided crystallization of the transcription regulator PaaR2 from Escherichia coli O157:H7. P. De Bruyn, M. Prolič-Kalinšek, A. Vandervelde, M. Malfait, Y. G.-J. Sterckx, F. Sobott, S. Hadži, E. Pardon, J. Steyaert & R. Loris (2021). Acta Cryst. F77, 374-384.

Structural and functional analysis of the SET3 histone deacetylase complex. A. A. Reyes, S. Fishbain & Y. He (2022). Acta Cryst. F78, 113-118.

Crystal structure determination of the halogenase CtcP from Streptomyces aureofaciens. L. Yin (2022). Acta Cryst. F78, 270-275.

Crystal structure of N-terminal degron-truncated human glutamine synthetase. M. F. Chek, S.-Y. Kim, T. Mori, H. Kojima & T. Hakoshima (2021). Acta Cryst. F77, 427-434.

Frequency distribution of space groups in soluble and membrane proteins and their complexes. R. K. Gaur (2021). Acta Cryst. F77, 187-191.

Crystal structure of enoyl-CoA hydratase from Thermus thermophilus HB8. S. Padavattan, S. Jos, H. Gogoi & B. Bagautdinov (2021). Acta Cryst. F77, 148-155.

Novel combined crystallization plate for high-throughput crystal screening and in situ data collection at a crystallography beamline. M. Liang, L. Yu, Z. Wang, H. Zhou, Y. Zhang, Q. Wang & J. He (2021). Acta Cryst. F77, 319-327.

Crystal structures of glycogen-debranching enzyme mutants in complex with oligosaccharides. M. Shen, X. Gong & S. Xiang (2021). Acta Cryst. F77, 420-426.

Structural and spectroscopic characterization of CO inhibition of [NiFe]-hydrogenase from Citrobacter sp. S-77. T. Imanishi, K. Nishikawa, M. Taketa, K. Higuchi, H. Tai, S. Hirota, H. Hojo, T. Kawakami, K. Hataguchi, K. Matsumoto, H. Ogata & Y. Higuchi (2022). Acta Cryst. F78, 66-74.

PAIREF: paired refinement also for Phenix users. M. Malý, K. Diederichs, J. Dohnálek & P. Kolenko (2021). Acta Cryst. F77, 226-229.

LrpCBA pilus proteins of gut-dwelling Ligilactobacillus ruminis: crystallization and X-ray diffraction analysis. A. Prajapati, A. Palva, I. von Ossowski & V. Krishnan (2021). Acta Cryst. F77, 238-245.

Structures of additional crystal forms of Satellite tobacco mosaic virus grown from a variety of salts. A. McPherson (2021). Acta Cryst. F77, 473-483.

Structural characterization of hexameric shell proteins from two types of choline-utilization bacterial microcompartments. J. M. Ochoa, O. Mijares, A. A. Acosta, X. Escoto, N. Leon-Rivera, J. D. Marshall, M. R. Sawaya & T. O. Yeates (2021). Acta Cryst. F77, 275-285.

Structural study of Legionella pneumophila effector DotY (Lpg0294), a component of the Dot/Icm type IV secretion system. I. Y. W. Chung & M. Cygler (2022). Acta Cryst. F78, 276-280.

Glycogen phosphorylase revisited: extending the resolution of the R- and T-state structures of the free enzyme and in complex with allosteric activators. D. D. Leonidas, S. E. Zographos, K. E. Tsitsanou, V. T. Skamnaki, G. Stravodimos & E. Kyriakis (2021). Acta Cryst. F77, 303-311.

Structure and activity of a thermally stable mutant of Acanthamoeba actophorin. S. Quirk & R. L. Lieberman (2022). Acta Cryst. F78, 150-160.

Crystal structures of human coronavirus NL63 main protease at different pH values. H. Gao, Y. Zhang, H. Jiang, X. Hu, Y. Zhang, X. Zhou, F. Zhong, C. Lin, J. Li, J. Luo & J. Zhang (2021). Acta Cryst. F77, 348-355.

Crystal structure of the PX domain of Vps17p from Saccharomyces cerevisiae. T. Obita, K. Inaka, D. Kohda & N. Maita (2022). Acta Cryst. F78, 210-216.

Nanocrystalline protein domains via salting-out. D. G. Greene, S. Modla, S. I. Sandler, N. J. Wagner & A. M. Lenhoff (2021). Acta Cryst. F77, 412-419.

Complex structure of the acyltransferase VinK and the carrier protein VinL with a pantetheine cross-linking probe. A. Miyanaga, R. Ouchi, F. Kudo & T. Eguchi (2021). Acta Cryst. F77, 294-302.

Improved resolution crystal structure of Acanthamoeba actophorin reveals structural plasticity not induced by microgravity. S. Quirk & R. L. Lieberman (2021). Acta Cryst. F77, 452-458.

Crystal structure of the complex of DNA with the C-terminal domain of TYE7 from Saccharomyces cerevisiae. W. Gui, L. Xue, J. Yue, Z. Kuang, Y. Jin & L. Niu (2021). Acta Cryst. F77, 341-347.

Preliminary results of neutron and X-ray diffraction data collection on a lytic polysaccharide monooxygenase under reduced and acidic conditions. G. C. Schröder, W. B. O'Dell, P. D. Swartz & F. Meilleur (2021). Acta Cryst. F77, 128-133.

Structural insights into the intermolecular interaction of the adhesin SdrC in the pathogenicity of Staphylococcus aureus. J. Wang, M. Zhang, M. Wang, J. Zang, X. Zhang & T. Hang (2021). Acta Cryst. F77, 47-53.

Sleuthing biochemical evidence to elucidate unassigned electron density in a CBL–SLAP2 crystal complex. L. E. Wybenga-Groot & C. J. McGlade (2021). Acta Cryst. F77, 37-46.

Structural investigation of a pyrano-1,3-oxazine derivative and the phenanthridinone core moiety against BRD2 bromodomains. A. H. Arole, P. Deshmukh, A. Sridhar & B. Padmanabhan (2022). Acta Cryst. F78, 119-127.

Crystal structure of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis. K. Sasamoto, T. Himiyama, K. Moriyoshi, T. Ohmoto, K. Uegaki, Y. Nishiya & T. Nakamura (2021). Acta Cryst. F77, 399-406.

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