most read articles

These are the most read articles in the last six months.

Using resources generated by the Seattle Structural Genomics Center for Infectious Disease (SSGCID) for training early career researchers. J. V. Early, C. L. Smith, O. A. Asojo & P. J. Myler (2025). Acta Cryst. F81, 222-225. 
Preparing for successful protein crystallization experiments. G. R. Budziszewski, V. Stojanoff & S. E. J. Bowman (2025). Acta Cryst. F81, 272-280. 
Sample purification and characterization of the α- and β-crustacyanin pigments from the American lobster for crystallographic and cryo-EM studies. M. C. Cedri, H. Bansia, A. Amici, T. Collet, P. Moretti, A. A. McCarthy, C. Mueller-Dieckmann, N. Raffaelli, T. Wang, A. des Georges & M. Cianci (2026). Acta Cryst. F82, 49-56. 
Crystallization and 1.6 Å resolution crystal structure of an acylated GLP-1/GIP analogue peptide. H. M. Mitchell, B. Nocek, E. J. Guinn & J. Y. Y. Heng (2026). Acta Cryst. F82, 114-124. 
The first report of structural analysis of a nucleic acid using crystals grown in space. S. Ando, M. Takahashi & J. Kondo (2025). Acta Cryst. F81, 95-100. 
Triosephosphate isomerase from Fasciola hepatica: high-resolution crystal structure as a drug target. G. Kontellas, D. J. Studholme, M. van der Giezen, D. J. Timson, J. A. Littlechild & M. N. Isupov (2025). Acta Cryst. F81, 381-387. 
Crystal structure of Methanococcus jannaschii dihydroorotase with substrate bound. J. Vitali, J. C. Nix, H. E. Newman & M. J. Colaneri (2026). Acta Cryst. F82, 23-31. 
Structures of pentatricopeptide repeat proteins. A. Pullakhandam, C. Cooper, I. D. Small & C. S. Bond (2026). Acta Cryst. F82, 101-113. 
LOPAC library screening identifies suramin as a TRIM21 binder with a unique binding mode revealed by crystal structure. Y. Kim, S. Knapp & A. Krämer (2025). Acta Cryst. F81, 101-107. 
CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection. J.-H. Schäfer, A. Calza, K. Hom, P. Damodar, R. Peng, N. Bogdanović, G. C. Lander, S. M. Stagg & M. A. Cianfrocco (2025). Acta Cryst. F81, 517-526. 
Structural insights into the binding mode of the hypnotic drug vornorexant to orexin receptors. M. Mima, C. Mishima-Tsumagari, M. Sakata-Sakuta, R. Iwaoka & K. Iwamoto (2026). Acta Cryst. F82, 201-207. 
Crystal structure of a short-chain dehydrogenase from Brucella ovis with apo and coenzyme NAD+-bound protomer chains. S. P. Zupko, A. T. Konstanty, S. J. Mayclin, R. Choi, D. Serbzhinskiy, E. Robles, V. Moses, L. K. Barrett, W. C. Van Voorhis, T. E. Edwards, P. J. Myler, A. T. Torelli, J. B. French & K. A. Hicks (2025). Acta Cryst. F81, 487-494. 
Optimized bacterial expression of a synthetic BRIL antibody. B. F. Cooper & G. L. Isom (2026). Acta Cryst. F82, 143-149. 
Structural insights into full-length human fascin1: a target for cancer treatment. L. Giraldo-Ruiz, I. Quereda-Moraleda, A. Grieco, J. Ruiz-Sanz, I. Luque & J. M. Martin-Garcia (2025). Acta Cryst. F81, 319-331. 
Crystal structure of a short-chain dehydrogenase from Burkholderia cenocepacia J2315 in complex with NADP+ and benzoic acid. K. K. J. Belfon, O. Beyer, J. Abendroth, D. M. Dranow, D. D. Lorimer, A. Abramov, Y. Latimore, C. Hamilton, A. Dawkins, I. Hinojosa, X. Martinez, S. Mirabel, M. Duncan, R. Womack, L. Hicks, Z. R. Turlington, T. E. Edwards, A. T. Torelli, K. A. Hicks & J. B. French (2024). Acta Cryst. F80, 348-355. 
Glucose-6-phosphate dehydrogenase and its 3D structures from crystallography and electron cryo-microscopy. S. Hanau & J. R. Helliwell (2024). Acta Cryst. F80, 236-251. 
First crystal structure of the DUF2436 domain of virulence proteins from Porphyromonas gingivalis. B. Kim, J. Hwang, S. Im, H. Do, Y.-S. Shim & J. H. Lee (2024). Acta Cryst. F80, 252-262. 
Cryo-EM structures of a MexB–MexY chimeric efflux pump reveal that large open clefts are intrinsic to the MexY porter domain. J. Wang, K. Tsutsumi, M. Hirose, R. Nakashima, T. Kato, K. Nishino, A. Nakagawa & E. Yamashita (2026). Acta Cryst. F82, 83-93. 
Co-crystal structure of Helicobacter pylori biotin protein ligase with biotinyl-5-ATP. J. P. Ayanlade, D. E. Davis, S. Subramanian, D. M. Dranow, D. D. Lorimer, B. Hammerson, P. J. Myler & O. A. Asojo (2025). Acta Cryst. F81, 11-18. 
Crystal structure of carbon–nitrogen hydrolase from Helicobacter pylori G27. A. Srivastava, J. P. Ayanlade, L. Suleiman, H. Udell, J. Abendroth, DJ. Lorimer, T. E Edwards, B. L. Staker, R. Zigweid, S. Subramanian, P. J. Myler, G. Chakafana & O. A. Asojo (2026). Acta Cryst. F82, 66-74. 
Structure of the imine reductase from Ajellomyces dermatitidis in three crystal forms. Corrigendum. M. Sharma, A. Cuetos, A. Williams, D. González-Martínez & G. Grogan (2026). Acta Cryst. F82, 41. 
Crystal structure of ATP-dependent DNA ligase from Rhizobium phage vB_RleM_P10VF. U. Rothweiler, H.-K. S. Leiros & A. Williamson (2025). Acta Cryst. F81, 249-254. 
Crystal structure of a tRNA acceptor-stem mimic at 1.94 Å resolution. Z. Liu, D. Bellini, F. Gorrec, A. Wagner, K. El Omari & J. D. Sutherland (2026). Acta Cryst. F82, 57-65. 
Serendipitous high-resolution structure of Escherichia coli carbonic anhydrase 2. M. R. Rankin & J. L. Smith (2025). Acta Cryst. F81, 47-52. 
Revisiting sodium phosphotungstate and ammonium molybdate as nonradioactive negative-staining agents for single-particle analysis. M. Gunkel, A. Macha & E. Behrmann (2024). Acta Cryst. F80, 356-362. 
Off-target binding of the histone deacetylase inhibitor vorinostat to carbonic anhydrase II and IX. M. C. Gulkis, J. T. Hodgkinson, C. P. Sele, W. Knecht, R. McKenna & S. Z. Fisher (2025). Acta Cryst. F81, 388-397. 
Single mutations to tyrosine or glutamate improve the crystallizability and crystal diffraction properties of a flexible two-domain protein. C. Geerds & H. H. Niemann (2026). Acta Cryst. F82, 4-13. 
Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody. S. Semwal, M. Karamolegkou, S. Flament, N. Raouraoua, K. Verstraete, A. Thureau, F. Wien, F. Bray, S. N. Savvides & J. Bouckaert (2025). Acta Cryst. F81, 19-29.