most read articles

These are the most read articles in the last six months.

Preparing research samples for safe arrival at centers and facilities: recipes for successful experiments. S. E. J. Bowman, J. Byrnes, S. Russi & C. M. Zimanyi (2024). Acta Cryst. F80, 165-172.

Glucose-6-phosphate dehydrogenase and its 3D structures from crystallography and electron cryo-microscopy. S. Hanau & J. R. Helliwell (2024). Acta Cryst. F80, 236-251.

Richard Alexander Pauptit 1954–2024. C. Dennis, D. Derbyshire, J. Jaeger & A. Riboldi-Tunnicliffe (2024). Acta Cryst. F80, 191-192.

Welcoming Alejandro Buschiazzo, Dorothee Liebschner and Stephen Muench as Co-editors of Acta Crystallographica F – Structural Biology Communications. J. Agirre, M. C. Nonato & M. J. van Raaij (2024). Acta Cryst. F80, 234-235.

Ternary structure of Plasmodium vivax N-myristoyltransferase with myristoyl-CoA and inhibitor IMP-0001173. C. Bolling, A. Mendez, S. Taylor, S. Makumire, A. Reers, R. Zigweid, S. Subramanian, D. M. Dranow, B. Staker, T. E. Edwards, E. W. Tate, A. S. Bell, P. J. Myler, O. A. Asojo & G. Chakafana (2024). Acta Cryst. F80, 269-277.

Multi-species cryoEM calibration and workflow verification standard. D. Bobe, M. Kopylov, J. Miller, A. P. Owji & E. T. Eng (2024). Acta Cryst. F80, 320-327.

Crystallization and crystallographic studies of human serine protease inhibitor (serpin) B9. T. Yan & A. Zhou (2024). Acta Cryst. F80, 286-293.

Tricks and tips for trips. M. J. van Raaij (2024). Acta Cryst. F80, 164.

Crystallographic fragment screen of the c-di-AMP-synthesizing enzyme CdaA from Bacillus subtilis. T. Garbers, P. Neumann, J. Wollenhaupt, A. Dickmanns, M. S. Weiss & R. Ficner (2024). Acta Cryst. F80, 200-209.

X-ray crystal structure of proliferating cell nuclear antigen 1 from Aeropyrum pernix. T. Yamauchi, M. Kikuchi, Y. Iizuka & M. Tsunoda (2024). Acta Cryst. F80, 294-301.

First crystal structure of the DUF2436 domain of virulence proteins from Porphyromonas gingivalis. B. Kim, J. Hwang, S. Im, H. Do, Y.-S. Shim & J. H. Lee (2024). Acta Cryst. F80, 252-262.

Structures of Brucella ovis leucine-, isoleucine-, valine-, threonine- and alanine-binding protein reveal a conformationally flexible peptide-binding cavity. G. Chakafana, R. Boswell, A. Chandler, K. A. Jackson, S. Neblett, T. Postal, S. Subramanian, J. Abendroth, P. J. Myler & O. A. Asojo (2024). Acta Cryst. F80, 193-199.

Human transforming growth factor β type I receptor in complex with kinase inhibitor SB505124. J. A. Rodriguez Buitrago, M. Landström & M. Wolf-Watz (2024). Acta Cryst. F80, 314-319.

The impact of exchanging the light and heavy chains on the structures of bovine ultralong antibodies. J. D. Clarke, A. Douangamath, H. Mikolajek, M. Bonnet-Di Placido, J. Ren, E. E. Fry, D. I. Stuart, J. A. Hammond & R. J. Owens (2024). Acta Cryst. F80, 154-163.

Crystal structure of guanosine 5′-monophosphate synthetase from the thermophilic bacterium Thermus thermophilus HB8. N. Nemoto, S. Baba, G. Kawai & G. Sampei (2024). Acta Cryst. F80, 278-285.

Preliminary X-ray diffraction and ligand-binding analyses of the N-terminal domain of hypothetical protein Rv1421 from Mycobacterium tuberculosis H37Rv. J. Park, Y. J. Cheon, Y. C. Jeong & K. S. Lee (2024). Acta Cryst. F80, 135-141.

High-resolution double vision of the allosteric phosphatase PTP1B. S. Sharma, T. Skaist Mehlman, R. S. Sagabala, B. Boivin & D. A. Keedy (2024). Acta Cryst. F80, 1-12.

Crystal structure of N-terminally hexahistidine-tagged Onchocerca volvulus macrophage migration inhibitory factor-1. A. D. Kimble, O. C. O. Dawson, L. Liu, S. Subramanian, A. Cooper, K. Battaile, J. Craig, E. Harmon, P. Myler, S. Lovell & O. A. Asojo (2024). Acta Cryst. F80, 328-334.

The structure of a pectin-active family 1 polysaccharide lyase from the marine bacterium Pseudoalteromonas fuliginea. J. K. Hobbs & A. B. Boraston (2024). Acta Cryst. F80, 142-147.

X-ray crystal structure of a designed rigidified imaging scaffold in the ligand-free conformation. M. P. Agdanowski, R. Castells-Graells, M. R. Sawaya, D. Cascio, T. O. Yeates & M. A. Arbing (2024). Acta Cryst. F80, 107-115.

Structure of the GDP-bound state of the SRP GTPase FlhF. A. Dornes, C.-N. Mais & G. Bange (2024). Acta Cryst. F80, 53-58.

Room-temperature serial synchrotron crystallography of the human phosphatase PTP1B. S. Sharma, A. Ebrahim & D. A. Keedy (2023). Acta Cryst. F79, 23-30.

Improving the diffraction quality of heat-shock protein 47 crystals. K. Kish, S. Cobell, N. Szapiel, C. Yan, J. A. Newitt, J. Tredup, I. Rodrigo, E. Tomasco, M. Gao, F. Marsilio, J. Haugner, D. Lipovšek, B. Deng, P. Bousquet, Y. Zhang, H. Schmidt & S. Sheriff (2024). Acta Cryst. F80, 302-313.

Crystal structures of NAD(P)H nitroreductases from Klebsiella pneumoniae. A. D. Kancherla, L. Liu, L. Tillery, R. Shek, J. K. Craig, A. J. Machen, S. Seibold, K. P. Battaile, S. Fradi, L. K. Barrett, S. Subramanian, P. Myler, W. C. Van Voorhis & S. Lovell (2024). Acta Cryst. F80, 173-182.

Protein expression, purification, crystallization and crystallographic studies of BPSL0741 from Burkholderia pseudomallei. N. F. Fadhar, P. K. Nyanasegran, M. Firdaus-Raih, S. Nathan, M. A. Jonet & C. L. Ng (2024). Acta Cryst. F80, 263-268.

Tetracycline-modifying enzyme SmTetX from Stenotrophomonas maltophilia. M. Malý, P. Kolenko, J. Stránský, L. Švecová, J. Dušková, T. Koval', T. Skálová, M. Trundová, K. Adámková, J. Černý, P. Božíková & J. Dohnálek (2023). Acta Cryst. F79, 180-192.

Crystal structure of glutamyl-tRNA synthetase from Helicobacter pylori. D. E. Davis, J. P. Ayanlade, D. T. Laseinde, S. Subramanian, H. Udell, D. J. Lorimer, D. M. Dranow, T. E. Edwards, P. J. Myler & O. A. Asojo (2024). Acta Cryst. F80, 335-340.

Cryo-EM sample preparation for high-resolution structure studies. L. Wang & C. M. Zimanyi (2024). Acta Cryst. F80, 74-81.

Online carbohydrate 3D structure validation with the Privateer web app. J. S. Dialpuri, H. Bagdonas, L. C. Schofield, P. T. Pham, L. Holland, P. S. Bond, F. Sánchez Rodríguez, S. J. McNicholas & J. Agirre (2024). Acta Cryst. F80, 30-35.

Structure of the outer membrane porin OmpW from the pervasive pathogen Klebsiella pneumoniae. C. Seddon, G. Frankel & K. Beis (2024). Acta Cryst. F80, 22-27.

Structures of Trichomonas vaginalis macrophage migratory inhibitory factor. A. Srivastava, A. Nair, O. C. O. Dawson, R. Gao, L. Liu, J. K. Craig, K. P. Battaile, E. K. Harmon, L. K. Barrett, W. C. Van Voorhis, S. Subramanian, P. J. Myler, S. Lovell, O. A. Asojo & R. Darwiche (2024). Acta Cryst. F80, 341-347.

The catalytic domains of Streptococcus mutans glucosyltransferases: a structural analysis. N. Schormann, M. Patel, L. Thannickal, S. Purushotham, R. Wu, J. L. Mieher, H. Wu & C. Deivanayagam (2023). Acta Cryst. F79, 119-127.

Structure of Plasmodium vivax N-myristoyltransferase with inhibitor IMP-1088: exploring an NMT inhibitor for antimalarial therapy. A. Mendez, C. Bolling, S. Taylor, S. Makumire, B. Staker, A. Reers, B. Hammerson, S. J. Mayclin, J. Abendroth, D. D. Lorimer, T. E. Edwards, E. W. Tate, S. Subramanian, A. S. Bell, P. J. Myler, O. A. Asojo & G. Chakafana (2025). Acta Cryst. F81, https://doi.org/10.1107/S2053230X24011348.

Crystal structure of the RNA-recognition motif of Drosophila melanogaster tRNA (uracil-5-)-methyltransferase homolog A. M. Witzenberger, R. Janowski & D. Niessing (2024). Acta Cryst. F80, 36-42.

Cryo-EM structure and functional analysis of the chromatin remodeler RSF. J. Zhang, H. Zhao, B. Zou, H. Li, S. Dong, J. Guan, C. Wang, W. Li, Y. Liu, Y. Chen, N. Rasheed & J. He (2024). Acta Cryst. F80, 125-134.

Revisiting sodium phosphotungstate and ammonium molybdate as nonradioactive negative-staining agents for single-particle analysis. M. Gunkel, A. Macha & E. Behrmann (2024). Acta Cryst. F80, 356-362.

Room-temperature serial synchrotron crystallography structure of Spinacia oleracea RuBisCO. M. Bjelčić, O. Aurelius, J. Nan, R. Neutze & T. Ursby (2024). Acta Cryst. F80, 117-124.

Crystal structures of the 3C proteases from Coxsackievirus B3 and B4. H. Jiang, C. Lin, J. Chang, X. Zou, J. Zhang & J. Li (2024). Acta Cryst. F80, 183-190.

Monomeric crystal structure of the vaccine carrier protein CRM₁₉₇ and implications for vaccine development. D. T. Gallagher, N. Oganesyan & A. Lees (2023). Acta Cryst. F79, 82-86.

Structural and biophysical characterization of the Borna disease virus 1 phosphoprotein. J. D. Whitehead, J. M. Grimes & J. R. Keown (2023). Acta Cryst. F79, 51-60.

Structural analysis of the FERM domain of human protein tyrosine phosphatase non-receptor type 21. H. S. Lee, B. Ku, H.-C. Shin & S. J. Kim (2024). Acta Cryst. F80, 148-153.

Enhancing the apo protein tyrosine phosphatase non-receptor type 2 crystal soaking strategy through inhibitor-accessible binding sites. S. M. Bester, R. Linwood, R. Kataoka, W.-I. Wu & T.-C. Mou (2024). Acta Cryst. F80, 210-219.

Crystal structure of adenylosuccinate lyase from the thermophilic bacterium Thermus thermophilus HB8. N. Nemoto, G. Kawai & G. Sampei (2023). Acta Cryst. F79, 278-284.

Structural and biochemical characterization of the M405S variant of Desulfovibrio vulgaris formate dehydrogenase. G. Vilela-Alves, R. Rebelo Manuel, N. Pedrosa, I. A. Cardoso Pereira, M. J. Romão & C. Mota (2024). Acta Cryst. F80, 98-106.

Expression, purification and crystallization of the photosensory module of phytochrome B (phyB) from Sorghum bicolor. S. M. Shenkutie, S. Nagano & J. Hughes (2024). Acta Cryst. F80, 59-66.

Crystal structure of the GDP-bound human M-RAS protein in two crystal forms. S. M. Bester, R. Abrahamsen, L. Rodrigues Samora, W.-I Wu & T.-C. Mou (2024). Acta Cryst. F80, 220-227.

High-resolution crystal structure of the Mu8.1 conotoxin from Conus mucronatus. E. Müller, C. M. Hackney, L. Ellgaard & J. P. Morth (2023). Acta Cryst. F79, 240-246.

The crystal structure of the human smacovirus 1 Rep domain. L. K. Limón, K. Shi, A. Dao, J. Rugloski, K. J. Tompkins, H. Aihara, W. R. Gordon & R. L. Evans III (2023). Acta Cryst. F79, 295-300.

AlphaFold and the future of structural biology. R. J. Read, E. N. Baker, C. S. Bond, E. F. Garman & M. J. van Raaij (2023). Acta Cryst. F79, 166-168.

Crystal structure of MbnF: an NADPH-dependent flavin monooxygenase from Methylocystis strain SB2. A. Stewart, P. Dershwitz, C. Stewart Jr, M. R. Sawaya, T. O. Yeates, J. D. Semrau, H. Zischka, A. A. DiSpirito & T. A. Bobik (2023). Acta Cryst. F79, 111-118.

Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature. T. Tandrup, L. Lo Leggio & F. Meilleur (2023). Acta Cryst. F79, 1-7.

Making your raw data available to the macromolecular crystallography community. L. M. J. Kroon-Batenburg (2023). Acta Cryst. F79, 267-273.

Purification and structure of luminal domain C of human Niemann–Pick C1 protein. L. Odongo, K. K. Zadrozny, W. E. Diehl, J. Luban, J. M. White, B. K. Ganser-Pornillos, L. K. Tamm & O. Pornillos (2023). Acta Cryst. F79, 45-50.

Drastic alterations in the loop structure around colchicine upon complex formation with an engineered lipocalin indicate a conformational selection mechanism. E. Jerschke, A. Eichinger & A. Skerra (2023). Acta Cryst. F79, 231-239.

The importance of international collaborations in science and structural biology. M. C. Nonato, M. J. van Raaij & J. Agirre (2024). Acta Cryst. F80, 116.

Using structural genomics depositions in undergraduate teaching of protein crystallography: everybody wins. J. Agirre (2023). Acta Cryst. F79, 274-275.

Structure of the imine reductase from Ajellomyces dermatitidis in three crystal forms. M. Sharma, A. Cuetos, A. Willliams, D. González-Martínez & G. Grogan (2023). Acta Cryst. F79, 224-230.

Crystal structure of the Rib domain of the cell-wall-anchored surface protein from Limosilactobacillus reuteri. Y. Xue, Z. Wu & X. Kang (2024). Acta Cryst. F80, 228-233.

Crystal structure of a GCN5-related N-acetyltransferase from Lactobacillus curiae. J. R. Fleming, F. Hauth, J. S. Hartig & O. Mayans (2023). Acta Cryst. F79, 217-223.

Structures of chloramphenicol acetyltransferase III and Escherichia coli β-ketoacylsynthase III co-crystallized with partially hydrolysed acetyl-oxa(dethia)CoA. A. B. Benjamin, L. M. Stunkard, J. Ling, J. N. Nice & J. R. Lohman (2023). Acta Cryst. F79, 61-69.

Biochemical and X-ray analyses of the players involved in the faRel2/aTfaRel2 toxin–antitoxin operon. L. Dominguez-Molina, A. Talavera, A. Cepauskas, T. Kurata, D. Echemendia-Blanco, V. Hauryliuk & A. Garcia-Pino (2023). Acta Cryst. F79, 247-256.

Beyond publishing: introducing Interviews with authors. M. C. Nonato, M. J. van Raaij & J. Agirre (2024). Acta Cryst. F80, 73.

Crystal structure of Prp16 in complex with ADP. T. B. Garbers, M. Enders, P. Neumann & R. Ficner (2023). Acta Cryst. F79, 200-207.

The structure of a tautomerase superfamily member linked to the type VI secretion system of Acinetobacter baumannii. G. Pankov, G. Mol Avelar, G. Buchanan, S. J. Coulthurst & W. N. Hunter (2023). Acta Cryst. F79, 8-16.

Structural and functional characterization of FabG4 from Mycolicibacterium smegmatis. X. Ran, P. Parikh, J. Abendroth, T. L. Arakaki, M. C. Clifton, T. E. Edwards, D. D. Lorimer, S. Mayclin, B. L. Staker, P. Myler & K. J. McLaughlin (2024). Acta Cryst. F80, 82-91.

Co-crystal structure of Helicobacter pylori biotin protein ligase with biotinyl-5-ATP. J. P. Ayanlade, D. E. Davis, S. Subramanian, D. M. Dranow, D. D. Lorimer, B. Hammerson, P. J. Myler & O. A. Asojo (2025). Acta Cryst. F81, https://doi.org/10.1107/S2053230X24012056.

Biochemical and structural characterization of a class A β-lactamase from Nocardia cyriacigeorgica. J. Feuillard, J. Couston, Y. Benito, E. Hodille, O. Dumitrescu & M. Blaise (2024). Acta Cryst. F80, 13-21.

The unbearable burden of peer review?. M. J. van Raaij (2024). Acta Cryst. F80, 52.

Shaping the future of Acta Crystallographica F: unveiling our vision. M. C. Nonato (2023). Acta Cryst. F79, 294.

A partially open conformation of an androgen receptor ligand-binding domain with drug-resistance mutations. S. K. Doamekpor, P. Peng, R. Xu, L. Ma, Y. Tong & L. Tong (2023). Acta Cryst. F79, 95-104.

Crystallization and biochemical studies of the NYN domain of human KHNYN. S. Hong & J. Choe (2024). Acta Cryst. F80, 67-72.

Crystal structure of the long Rib domain of the LPXTG-anchored surface protein from Limosilactobacillus reuteri. Y. Xue, Z. Wu & X. Kang (2024). Acta Cryst. F80, 92-97.

Crystal structure of the engineered endolysin mtEC340M. J.-M. Wang, S.-H. Seok, W.-S. Yoon, J.-H. Kim & M.-D. Seo (2023). Acta Cryst. F79, 105-110.

Structure of pyridoxal 5′-phosphate-bound D-threonine aldolase from Chlamydomonas reinhardtii. Y. Hirato, M. Goto, T. Mizobuchi, H. Muramatsu, M. Tanigawa & K. Nishimura (2023). Acta Cryst. F79, 31-37.

Characterization of a family I inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1. J. Moorefield, Y. Konuk, J. O. Norman, J. Abendroth, T. E. Edwards, D. D. Lorimer, S. J. Mayclin, B. L. Staker, J. K. Craig, K. F. Barett, L. K. Barrett, W. C. Van Voorhis, P. J. Myler & K. J. McLaughlin (2023). Acta Cryst. F79, 257-266.

Structural and enzymatic characterization of the sialidase SiaPG from Porphyromonas gingivalis. W.-B. Dong, Y.-L. Jiang, Z.-L. Zhu, J. Zhu, Y. Li, R. Xia & K. Zhou (2023). Acta Cryst. F79, 87-94.

Crystal structure and biophysical characterization of IspD from Burkholderia thailandensis and Mycobacterium paratuberculosis. P. G. Pierce, B. E. Hartnett, T. M. Laughlin, J. M. Blain, S. J. Mayclin, M. J. Bolejack, J. B. Myers, T. W. Higgins, D. M. Dranow, A. Sullivan, D. D. Lorimer, T. E. Edwards, T. J. Hagen, J. R. Horn & P. J. Myler (2024). Acta Cryst. F80, 43-51.

Structure of a superoxide dismutase from a tardigrade: Ramazzottius varieornatus strain YOKOZUNA-1. K.-S. Sim & T. Inoue (2023). Acta Cryst. F79, 169-179.

Private glycan investigations and public video content. M. J. van Raaij (2024). Acta Cryst. F80, 28-29.

Introduction to the virtual thematic issue on room-temperature biological crystallography. R. A. Steiner (2023). Acta Cryst. F79, 79-81.

Structural basis for the development of potential inhibitors targeting FadD23 from Mycobacterium tuberculosis. M. Yan, M. Ma, R. Chen, Y. Cao, W. Zhang & X. Liu (2023). Acta Cryst. F79, 208-216.

Direct relationship between dimeric form and activity in the acidic copper–zinc superoxide dismutase from lemon. R. A. Utami, H. Yoshida, L. H. Kartadinata, V. A. Abdillah, C. R. Faratilla, D. S. Retnoningrum & W. T. Ismaya (2023). Acta Cryst. F79, 301-307.

Crystal structure of CmnB involved in the biosynthesis of the nonproteinogenic amino acid L-2,3-diaminopropionic acid. S.-I. Toh, C.-L. Lo & C.-Y. Chang (2023). Acta Cryst. F79, 193-199.

Bacterial structural genomics target enabled by a recently discovered potent fungal acetyl-CoA synthetase inhibitor. N. D. DeBouver, M. J. Bolejack, T. E. Esan, D. J. Krysan, T. J. Hagen & J. Abendroth (2023). Acta Cryst. F79, 137-143.

Crystal structures of QseE and QseG: elements of a three-component system from Escherichia coli. K. Matsumoto, Y. Fukuda & T. Inoue (2023). Acta Cryst. F79, 285-293.

Crystal structure of the C-terminal domain of the plant-specific microtubule-associated protein Spiral2. M. Ohno, Y. Higuchi & I. Hayashi (2023). Acta Cryst. F79, 17-22.

An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1.4. T. Y. H. Bui, P. Dedecker & L. Van Meervelt (2023). Acta Cryst. F79, 38-44.

Crystal structure of MAB_4123, a putative flavin-dependent monooxygenase from Mycobacterium abscessus. K. L. Ung, C. Poussineau, J. Couston, H. M. A. B. Alsarraf & M. Blaise (2023). Acta Cryst. F79, 128-136.

A triumvirate of Section Editors moving Structural Biology Communications ahead. M. van Raaij (2023). Acta Cryst. F79, 276-277.

Expression, purification and crystallization of N-acetyl-(R)-β-phenylalanine acylases derived from Burkholderia sp. AJ110349 and Variovorax sp. AJ110348 and structure determination of the Burkholderia enzyme. Y. Kato, H. Kawasaki, T. Nakamatsu, N. Matsuda & R. Natsume (2023). Acta Cryst. F79, 70-78.

Crystal structure of thermostable acetaldehyde dehydrogenase from the hyperthermophilic archaeon Sulfolobus tokodaii. S. Mine, M. Nakabayashi & K. Ishikawa (2023). Acta Cryst. F79, 159-165.

Crystal structure of a three-tetrad, parallel, K⁺-stabilized human telomeric G-quadruplex at 1.35 Å resolution. E. V. Chen, J. M. Nicoludis, B. M. Powell, K. S. Li & L. A. Yatsunyk (2023). Acta Cryst. F79, 144-150.

Crystal structure of a putative 3-hydroxypimelyl-CoA dehydrogenase, Hcd1, from Syntrophus aciditrophicus strain SB at 1.78 Å resolution. D. M. Dinh, L. M. Thomas & E. A. Karr (2023). Acta Cryst. F79, 151-158.

Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody. S. Semwal, M. Karamolegkou, S. Flament, N. Raouraoua, K. Verstraete, A. Thureau, F. Wien, F. Bray, S. N. Savvides & J. Bouckaert (2025). Acta Cryst. F81, https://doi.org/10.1107/S2053230X24012159.

Crystallization and preliminary X-ray crystallographic studies of AfeH from Acimetobacter sp. DL-2. H. Tang, X. Zhang, Y. Duan & Y. Huang (2025). Acta Cryst. F81, https://doi.org/10.1107/S2053230X24012275.

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