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![[Figure 1]](jo5121fig1mag.jpg)
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Figure 1
Optical phenomena occurring in a protein crystal. (a) Comparison of in-solution (orange curve) and in crystallo (green curve, with a non-null non-flat baseline) AS spectra. (b) Representation of several optical phenomena occurring in crystals that can contribute to the background: reflectivity (green rays), refraction (orange rays), which does not per se cause photon loss, and Rayleigh scattering (blue rays), which causes photon loss. (c) Schematic representation of a single-crystal microspectrophotometry setup. The crystal is mounted in a loop and surrounded by its mother liquor. The focal cones of the upstream (top right, focusing the incident beam) and downstream (to the spectrophotometer, bottom left) objectives are shown in grey. The downstream focal cone is larger than the upstream one to mitigate the displacement of the focal-point effect described by von Stetten et al. (2015  ). |
![[Figure 1]](jo5121fig1.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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