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![[Figure 2]](jo5121fig2mag.jpg)
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Figure 2
The icAS spectra of different crystals of the Cerulean fluorescent protein, at several points after they were soaked from pH 8.0 to pH 4.0 (red to blue–green). (a) Without correction and (b) after constant-baseline correction and smoothing. (c) Example of baseline and scattering baseline correction of the red spectrum from (a), with the three segments used to fit the baseline: red-side baseline segment (cyan), peakless segment (magenta) and near-UV segment (lime). (d) Baseline and scattering corrected spectra, scaled according to their absorption at 430 nm for comparison purposes. The correction separates the red and yellow spectra from the dark-green and light-green spectra. The inset shows the detail of the peak summit, 390 to 450 nm. |
![[Figure 2]](jo5121fig2.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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