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![[Figure 4]](jo5121fig4mag.jpg)
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Figure 4
Time-resolved icOS data in the GUI. (a) Series of time-resolved AS spectra, collected on BR crystals, after exposure to a 560 nm nanosecond laser pulse, raw. (b) Constant-baseline corrected and smoothed spectra, with laser trace removed. (c) Series of light − dark difference spectra. For panels (a)–(c), the colour ramps from red to blue as the reaction progresses, and spectra are recorded at 3 µs, 10 µs, 30 µs, 100 µs, 300 µs, 1 ms, 3 ms, 10 ms, 30 ms, 100 ms, 300 ms and 1 s. (d) Absorption at 400 nm (M state of BR), as taken from the spectra presented in (b), with the Hill equation fitted to data points from 10 to 1000 µs (pale-blue line). (e) Absorption spectrum recorded 3 µs after the actinic laser pulse, in which all dips in absorbance are identified by red dots. The largest dip in absorbance is identified by green dots and corresponds to the tail of the nanosecond laser pulse. (f) Confidence plot of the 300 µs TR-icOS spectrum; each data point is plotted from orange (low confidence) to green (good confidence). |
![[Figure 4]](jo5121fig4.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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