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 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
accessA simple goniometer-compatible flow cell for serial synchrotron X-ray crystallography. Corrigendum
aDepartment of Chemistry and Molecular Biology, University of Gothenburg, Medicinaregatan 9C, 40530 Gothenburg, Sweden, bMAX IV Laboratory, Lund University, Fotongatan 2, 224 84 Lund, Sweden, cDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom, and dMacromolecular Crystallography Group, Helmholtz-Zentrum Berlin, Albert-Einstein-Strasse 15, 12489 Berlin, Germany
*Correspondence e-mail: [email protected], [email protected]
Keywords: serial synchrotron X-ray crystallography; macromolecular crystallography; cytochrome c oxidase; goniometer-compatible flow cells.
In the article by Ghosh et al. (2023
), the microcrystal preparations used when measuring the absorption spectra shown in Fig. 5(b) were not accurately described. Microcrystalline samples were reduced by the addition of dithiothreitol and mixed with a photocage (Sandelin et al., 2024), and the absorption spectrum for the reduced state was recorded. The sample was translated, a laser flash of 355 nm was used to release dioxygen, and an absorption spectrum for the oxidized state was recorded after a delay of 1 ms. The absorption contribution of the photocage was subtracted from both spectra. Spectra from detergent-solubilized samples of cytochrome c oxidase (CcO) [Fig. 5(a)] were prepared as described: the oxidized spectrum is recorded from CcO samples as isolated, the reduced spectrum is recorded after treatment with sodium dithionite under anaerobic conditions, and no photocage was used in those measurements.
References
Ghosh, S., Zorić, D., Dahl, P., Bjelčić, M., Johannesson, J., Sandelin, E., Borjesson, P., Björling, A., Banacore, A., Edlund, P., Aurelius, O., Milas, M., Nan, J., Shilova, A., Gonzalez, A., Mueller, U., Brändén, G. & Neutze, R. (2023). J. Appl. Cryst. 56, 449–460. Web of Science CrossRef CAS IUCr Journals Google Scholar
Sandelin, E., Johannesson, J., Wendt, O., Brändén, G., Neutze, R. & Wallentin, C. (2024). Photochem. Photobiol. Sci. 23, 839–851. Web of Science CSD CrossRef CAS PubMed Google Scholar
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