journal menu
![[Figure 2]](jo5136fig2mag.jpg)
|
|
Figure 2
Schematic representation of preparation of segmentally deuterated ER-60 and SDS-PAGE analysis of the first- and second-step ligation reactions. (a) In the first step, the bb′ and a′ domains were ligated using OaAEP to generate bb′-a′. In the second step, the a domain was ligated to bb′-a′ to reconstruct full-length ER-60. (b) SDS-PAGE of the two-step ligation reactions. First step: ligation of bb′ and a′ domains. Second step: ligation of the a domain to bb′-a′. Lane 1: reaction mixture without OaAEP. Lane 2: reaction mixture with OaAEP. |
![[Figure 2]](jo5136fig2.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
Open 
access
access
