On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies

Lieske, J.; Cerv, M.; Kreida, S.; Komadina, D.; Fischer, J.; Barthelmess, M.; Fischer, P.; Pakendorf, T.; Yefanov, O.; Mariani, V.; Seine, T.; Ross, B.H.; Crosas, E.; Lorbeer, O.; Burkhardt, A.; Lane, T.J.; Guenther, S.; Bergtholdt, J.; Schoen, S.; Törnroth-Horsefield, S.; Chapman, H.N.; Meents, A.

doi:10.1107/S2052252519007395

Figure 1
Chip designs for the on-chip crystallization of proteins. (a) Roadrunner I chips have overall dimensions of 4.5 × 2.5 mm and up to 10 000 micro-pores, and are equipped with a standard magnetic base, making them compatible with data collection on most MX synchrotron beamlines. (b) Roadrunner II chips are much larger, with overall dimensions of 32 × 12 mm, and provide up to 300 000 micro-pores for crystals. They are glued onto an aluminium support frame (shown in light gray), which is mounted on a standard magnetic mount (shown in black). Room-temperature data collection with Roadrunner II chips requires a special scanning stage with a long travel range of more than 32 mm, such as the Roadrunner II goniometer (Tolstikova et al., 2019