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Figure 5
Procedure for on-chip ligand-soaking experiments. (a) Crystals are either grown on Roadrunner I chips or 1–3 µl of batch-grown crystals are applied to the chip. Under a stream of humidified air, the mother liquor is removed from the chip through the pores. (b) 2–3 µl of ligand-soaking solution is applied to the crystals. (c) One well of the crystallization chamber is filled with the appropriate reservoir solution. The chip is installed in the crystallization chamber and incubated for the desired time period. (d) The soaking solution is removed through the pores of the chip and crystals are either measured at room temperature or the chip is cooled in liquid nitrogen for cryogenic data collection.

Volume 6| Part 4| July 2019| Pages 714-728
ISSN: 2052-2525