On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies

Lieske, J.; Cerv, M.; Kreida, S.; Komadina, D.; Fischer, J.; Barthelmess, M.; Fischer, P.; Pakendorf, T.; Yefanov, O.; Mariani, V.; Seine, T.; Ross, B.H.; Crosas, E.; Lorbeer, O.; Burkhardt, A.; Lane, T.J.; Guenther, S.; Bergtholdt, J.; Schoen, S.; Törnroth-Horsefield, S.; Chapman, H.N.; Meents, A.

doi:10.1107/S2052252519007395

Figure 6
Exemplary results of crystallization experiments for proteinase K (a, b) and thermolysin (c, d) using the same crystallization conditions for conventional hanging-drop crystallization (a, c) and on-chip crystallization on Roadrunner I chips (b, d). The proteinase K crystals are around 30 µm in size and appeared overnight. The thermolysin crystals needed 1–2 days to reach a maximal size of ∼35 × 150 µm. The scale bars at the bottom right corners each have a length of 200 µm. The different background colors in the pictures showing on-chip crystallization stem from the different polylactic acid colors used for 3D-printing of the crystallization chambers.

IUCrJ

Volume 6| Part 4| July 2019| Pages 714-728

ISSN: 2052-2525

https://doi.org/10.1107/S2052252519007395

BIOLOGY | MEDICINE

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