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Figure 1
Data acquisition and image processing. (a) Typical electron micrograph as used for particle picking and image processing with RELION-3. (b) 2D class averages of complex IV with bound hemes visible as a single bright dot in the first two averages. (c) Fourier shell correlation and 0.143 cutoff. Refinement resulted in a map resolution of 5.5 Å. Black, corrected FSC; blue, masked FSC; green, unmasked FSC; red, phase-randomized FSC. All images were displayed within RELION-3. (d) SDS–PAGE of the fraction used for cryo-EM. (e) The same fraction on a blue native PAGE. Complex IV labeled as COX. (f) Local resolution map from RELION-3.

IUCrJ
Volume 6| Part 4| July 2019| Pages 773-780
ISSN: 2052-2525
https://doi.org/10.1107/S2052252519007486