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Figure 5
Fatty-acid binding measured by the displacement of a fluorescent probe (1,8-ANS) bound to human EPDR1. Fatty-acid binding to human EPDR1 was inferred by measuring the three fatty-acid (C6, caproic acid; C12, lauric acid; C18, stearic acid) concentrations necessary to replace 50% of 1,8-ANS (IC50). Note that the C6 and C12 displacement studies were performed in 0.5% ethanol buffer and that the C18 displacement study was performed in 2% ethanol buffer owing to the limited solubility of C18 in water.

IUCrJ
Volume 6| Part 4| July 2019| Pages 729-739
ISSN: 2052-2525
https://doi.org/10.1107/S2052252519007668