Toward G protein-coupled receptor structure-based drug design using X-ray lasers

Ishchenko, A.; Stauch, B.; Han, G.W.; Batyuk, A.; Shiriaeva, A.; Li, C.; Zatsepin, N.; Weierstall, U.; Liu, W.; Nango, E.; Nakane, T.; Tanaka, R.; Tono, K.; Joti, Y.; Iwata, S.; Moraes, I.; Gati, C.; Cherezov, V.

doi:10.1107/S2052252519013137

Figure 4
Ligand exchange in 5-HT_2B and A_2AAR. (a, b) Ligand-exchange experiment in LCP crystallization plates with 5-HT_2B. The transient ligand serotonin was exchanged for ERG (a) and DHE (b). The images were taken under cross-polarized light. No crystals appeared in control experiments (insets) set up without adding ligands to the precipitant solution. (c, d) Ligand-exchange experiment in glass syringes with A_2AAR. The transient ligand LUF5834 is exchanged for ZM241385. (c) LCP string immersed in precipitant solution in a glass syringe. (d) LCP sample titrated with 7.9 MAG before loading into an LCP injector.

IUCrJ

Volume 6| Part 6| November 2019| Pages 1106-1119

ISSN: 2052-2525

https://doi.org/10.1107/S2052252519013137

BIOLOGY | MEDICINE

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