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Figure 2
Structures of the three model systems from grids prepared on the TrEM setup. To test our ability to make high-quality EM grids by spraying proteins on a fast-moving plunging grid, three samples were tested and data are shown with representative micrographs and 3D reconstructions: (a, d) E. coli ribosome (0.72 µM in 50 mM HEPES pH 7.5, 100 mM KAc, 8 mM MgAc2), (b, e) apoferritin [30 µM (24-mer) in 20 mM HEPES pH 7.5, 150 mM NaCl], and (c, f) porcine thin filaments [5 µM (actin monomer) in 10 mM MOPS pH 7, 50 mM KAc, 3 mM MgCl2, 1 mM EGTA]. The scale bars in (a), (b) and (c) represents 50 nm. The scale bars in (d), (e) and (f) represents 5 nm.

Volume 6| Part 6| November 2019| Pages 1024-1031
ISSN: 2052-2525