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Figure 4
Rapid mixing of samples on the TrEM setup. (a) Representative micrograph with thin filaments blotted and apoferritin sprayed on the subsequent plunged grid. (b) Representative micrograph from the rapid mixing of apoferritin and thin filaments, and direct spraying onto the EM grid. (c) Four representative images of myosin S1 decorated filaments after the rapid mixing (∼15 ms) of 0, 8, 80 and 800 µM MgATP showing decoration consistent with that predicted by kinetic modelling (0, 10, 70 and 99%, respectively).

Volume 6| Part 6| November 2019| Pages 1024-1031
ISSN: 2052-2525