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Figure 2
Single-particle analysis of aldolase without beam-tilt correction. (a) Representative micrographs with minimal (left) and obvious (left) beam-tilt-induced objective astigmatism. Inset: cropped power spectrum. The scale bar is 100 nm. (b) Histogram of CTF resolution limits across the data set using CTFFIND4. (c) Representative 2D class averages calculated using RELION. The scale bar is 200 Å. (d) 3D classification results for selected particles after 2D classification. Dashed boxes indicate classes with particles used for subsequent 3D refinement. (e) Sharpened reconstruction after 3D refinement using RELION filtered to 4.9 Å resolution. (f) FSC curves for final reconstruction.

Volume 7| Part 6| November 2020| Pages 1179-1187
ISSN: 2052-2525