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Figure 4
Iterative CTF refinement with particle polishing improves the overall resolution to 2.8 Å. (a) Initial 3D structure at 4.9 Å resolution. Following the first CTF refinement and 3D refinement to obtain a structure at 3.8 Å resolution (b), continued CTF refinements alongside Bayesian particle polishing allowed resolution and B-factor improvements (c, d, e), ultimately allowing the determination of a 2.8 Å resolution structure (e). (f) FSC curves for 3D reconstructions from (a) to (e).

IUCrJ
Volume 7| Part 6| November 2020| Pages 1179-1187
ISSN: 2052-2525