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Figure 2
3D reconstruction of the 80S ribosome (data set EMPIAR-10028). (a) Surface representation of the low-pass-filtered ribosome after power-spectrum adjustment with a Gaussian high-pass filter. The structure has multiple flexible surface components and there is a residual ratchet movement of the 40S subunit (the left part of the structure in the orientation shown). (b) A generous mask used during structure refinement fills 11.5% of the volume. (c) A tight mask that typically would be used for resolution assessment fills 6.5% of the volume. (d) A very tight mask following the outline of the structure and eliminating the most flexible regions and any solvent noise fills 0.6% of the volume.

IUCrJ
Volume 7| Part 6| November 2020| Pages 995-1008
ISSN: 2052-2525
https://doi.org/10.1107/S2052252520011574