view article
Figure 3
The influence of a mask on FSC and mFSC resolution estimation. Black: mFSC without a mask; resolution 124 pixels (3.89 Å), CI (not shown) 123 pixels (3.92 Å). Solid red: FSC using a generous mask [Fig. 2[link](b)]; resolution 144 pixels (3.35 Å). Solid blue: mFSC using a generous mask [Fig. 2[link](b)]; resolution 143 pixels (3.37 Å). Solid light blue: CI of the mFSC; resolution 142 pixels (3.40 Å). Dotted red: FSC using a tight mask [Fig. 2[link](c)]; resolution 151 pixels (3.19 Å). Dotted blue: mFSC using a tight mask [Fig. 2[link](c)]; resolution 147 pixels (3.28 Å). Dotted light blue: CI of the mFSC; resolution 147 pixels (3.28 Å). Dashed red: FSC using a very tight mask [Fig. 2[link](d)]; resolution 171 pixels (2.82 Å). Dashed blue: mFSC using a very tight mask [Fig. 2[link](d)]; resolution 154 pixels (3.13 Å). Dashed light blue: CI of the mFSC; resolution 152 pixels (3.17 Å). The horizontal line marks the t = 0.143 resolution-cutoff threshold. All mFSC curves were computed with σg = 1 and one-sided confidence interval (CI) at α = 1%.

IUCrJ
Volume 7| Part 6| November 2020| Pages 995-1008
ISSN: 2052-2525
https://doi.org/10.1107/S2052252520011574