Figure 3
2D [1H,15N]-HSQC spectra of d-cGrx1 and m-cGrx1 recorded at 298 K on a Bruker Avance 800 MHz NMR spectrometer. (a) d-cGrx1. (b) m-cGrx1. (c, d) 2D [1H,15N]-HSQC spectra of cGrx1 with and without DTT. (c) d-cGrx1 in the absence of DTT is shown in blue and d-cGrx1 in the presence of DTT (5 mM) is shown in red.(d) m-cGrx1 in the absence of DTT is shown in blue and m-cGrx1 in the presence of DTT (5 mM) is shown in red. |