Figure 5
Deuterium uptake of d-cGrx1 and m-cGrx1. (a) The monomeric structures of d-cGrx1 and m-cGrx1 colored according to the differences in relative deuteration levels. Shades of red or blue reflect higher or lower deuterium uptake in the presence and absence of TCEP, respectively. (b, c) Uptake curves of selected peptides of d-cGrx1 and m-cGrx1. The observed relative deuterium uptake for each peptide, time point and condition were calculated and plotted against the labeling time. Error bars represent the average standard deviation observed across time points and replicates. |