Figure 3
(a) A chemical diagram of an N-ethylmaleimide-conjugated cysteine residue. (b) The 2Fo − Fc electron-density map contoured at the 0.8σ level (purple mesh) for N-ethylmaleimide-conjugated Cys156 in structure II. (c) The catalytic site in structure II. Possible hydrogen bonds are shown as blue dashed lines; the distance between Cys145 and His41 is shown as a black dotted line. The 2Fo − Fc electron-density map contoured at the 1.5σ level is shown as a purple mesh. All distances are given in ångströms. |