Figure 1
The crystal structures of McjD (a), SERCA (b) and RNAse A (c) were solved by vanadium phasing (top panel). The structures are shown as cartoons and the membrane for McjD and SERCA is depicted in pink. Middle panel: the phased anomalous difference Fourier maps obtained are drawn in magenta and contoured at 7σ for McjD and RNAse A and at 11σ for SERCA. Bottom panel: initial experimental electron-density maps were obtained from the CRANK2 pipeline and contoured at 1σ (after density modification and initial model building). |