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Figure 1
Purification and characterization of AcrIC9 in solution. (a) Schematic diagrams of the type IC CRISPR–Cas system and the target for AcrIC9. The crRNA that acts as a scaffold for the type IC cascade is shown by the red line. (b) A table summarizing the ten AcrIC families identified so far. The AcrIC9 characterized in this study is in red. (c) A SEC profile of AcrIC9. The inset is a photograph of an SDS–PAGE gel loaded with extracts from the SEC peak fractions. The corresponding SEC fractions are indicated by the black arrows. M indicates the protein size marker. (d) A graphical representation of the data obtained by using SEC–MALS (LS, light scattering). Below this there is experimental analysis of the results obtained from the MALS analysis of (e) the first peak and (f) the second peak. The experimental MALS data (red line) are plotted as the SEC elution volume (x axis) versus the absolute molecular mass (y axis) distribution on the SEC chromatogram (black) at 280 nm.

IUCrJ
Volume 10| Part 5| September 2023| Pages 624-634
ISSN: 2052-2525
https://doi.org/10.1107/S2052252523007236