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Figure 3
The tentative structure of the AcrIC9 dimer in solution. (a) A table summarizing the results using the PISA server. (b) Crystallographic packing symmetry via PyMOL showing the three AcrIC9 molecules (A, B and C) found in the ASU. Their other symmetries are labeled with ′ or ′′. (c) A schematic diagram of the putative dimeric structure of the B/B′ complex. (d) Details of the interactions in the B/B′ complex. (e) A schematic diagram of the putative dimeric structure of the C′/C′′ complex (Cys69: the residue involved in di­sulfide bond formation). (f) SEC profiles of wild-type AcrIC9 and various mutations of the putative interface binding sites. (g) A graphical representation of the SEC–MALS data for the putative interface binding sites of the various mutants (D33W, R37W and C69A). The first and second peaks generated for the dimer and monomer of AcrIC9 are indicated by the black arrows. (h) Experimental analysis of the MALS results for the SEC peaks generated from the C69A mutant. The experimental MALS data (red line) are plotted as the SEC elution volume (x axis) versus the absolute molecular mass (y axis) distribution on the SEC chromatogram (black) at 280 nm.

IUCrJ
Volume 10| Part 5| September 2023| Pages 624-634
ISSN: 2052-2525
https://doi.org/10.1107/S2052252523007236