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Figure 4
Direct interaction between AcrIC9 and the type IC cascade. (a) Interaction analysis between AcrIC9 and the type IC cascade by using SEC. The blue, black and red lines indicate AcrIC9, the cascade, and a mixture of AcrIC9 and the cascade, respectively. (b) SDS–PAGE gels produced after loading the SEC fractions of the two peaks (8–10 ml and 17–22 ml) from the mixed sample (the cascade + AcrIC9) shown in (a). The positions of each cascade subunit are indicated on the gel. AcrIC9 that co-migrated with the cascade is indicated by the red arrow. The corresponding fractions loaded onto the SDS–PAGE gels are indicated by the black and green bars. (c) Binding of AcrlC9 to the cascade complex captured on an NTA chip by using SPR. The cascade complex was captured on an NTA chip and AcrlC9 was bound to the cascade complex in the concentration range from 8 to 125 nM.

IUCrJ
Volume 10| Part 5| September 2023| Pages 624-634
ISSN: 2052-2525
https://doi.org/10.1107/S2052252523007236