Figure 5
Evidence showing that AcrIC9 binds directly to Cas7. (a), (b) Interaction analysis between (a) monomeric or (b) dimeric AcrIC9 and cascade subunit Cas7 via SEC. The black, red and blue lines indicate Cas7, a mixture of AcrIC9 and Cas7, and AcrIC9, respectively. The SDS–PAGE gels produced for the mixture of AcrIC9 and Cas7 are provided under the SEC profiles. The loaded SEC fractions on the SDS–PAGE gels are indicated by black and green bars, while the co-migrated AcrIC9 band is indicated by the red arrow. |