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Figure 2
(a) Rendering of the 60S model used as a template for 2DTM. Omitted atoms are shown in gray. (b) Slice through the simulated density. (c) The micrograph shown in Fig. 1[link](d) overlaid with 2DTM detections of the 60S subunit. (d) Magnified region of the cross-correlation maximum intensity projection (MIP), showing three distinct peaks. (e), (f) 3D plot of regions of the MIP around two of the peaks shown in (d). (g), (h) Number of 60S detections per imaged area in micrographs from CEMOVIS (g) and FIB-milling (h) sections plotted against the sample thickness estimated from CTF fitting. (i) Box plot of z-scores after the initial search with the 60S template in CEMOVIS or FIB-milled samples. (j) Box plot of SNRs after refinement of 60S detections in CEMOVIS or FIB-milled samples. The CEMOVIS data were collected as part of this study; the data from FIB-milled lamellae were collected by Lucas & Grigorieff (2023)BB22 (EMPIAR-11544).

IUCrJ
Volume 12| Part 4| July 2025| Pages 502-510
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525005196