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Figure 3
(a) Isosurface rendering of a 3D reconstruction from 60S detections in CEMOVIS sections. Parts of the density that were included in the template are shown in gray; parts that were omitted are shown in red. (b) Slice through the density of a 3D reconstruction from detections of 60S subunit in CEMOVIS. Compared with Fig. 2[link](b), there is density in the areas that were omitted from the template. (c) FSC calculated for the density in the central box of the map that was omitted from the template. The FSC between half-maps drops below 0.143 at 3.1 Å, while the FSC between map and model drops below 0.5 at 3.3 Å. (d) Close-up render of residues 2812–2814 of chain BB (25S ribosomal RNA) in PDB ID 6q8y, together with an isosurface of the reconstructed map, filtered to 3.2 Å and sharpened with a B factor of −75 Å2. The isosurface mesh was masked at a distance of 2 Å from the shown model. (e) Isosurface render of the density attributed to subunit L34A. The map was low-pass filtered at 3.5 Å and sharpened with a B factor of −30 Å2. The mesh was masked at a distance of 2 Å from the model of L34A. (f) Cartoon representation of subunit L34A. (g) Close-up render of residues 9–15 of chain BN (ribosomal protein L34A) in PDB ID 6q8y, together with an isosurface of the reconstructed map, filtered to 3.5 Å and sharpened with a B factor of −75 Å2. The isosurface mesh was masked at a distance of 2 Å from the shown model.

IUCrJ
Volume 12| Part 4| July 2025| Pages 502-510
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525005196