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Figure 3
Heme site structures of DtpB. (a) Dry heme site in the SFX ferric resting state structure devoid of any distal pocket axial ligand. (b) NO-bound heme site obtained at SACLA using the NO photocage 100 µs after 30 µJ laser illumination. (c) Heme site from the 100 µs 100 µJ structure showing the multiple conformations of Asn245 evident in some higher occupancy NO-bound states. (d) Overlay of structures shown in (a) yellow, dry heme site; (b) green, NO-bound 100 µs, 30 µJ; and (c) pink, NO-bound 100 µs, 100 µJ. Note a small shift in main-chain position for Asn245 in the dry heme site structure. In all panels chain A is shown.

IUCrJ
Volume 12| Part 5| September 2025| Pages 582-594
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525006645