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Figure 7
2Fo − Fc electron density maps contoured at 1σ for a representative heme group (chain C) of DtpB with different laser powers and time delay parameters obtained using fixed target SFX at SACLA. Clear electron density for the bound NO molecule is evident at 10 ms using 10 µJ laser power with an occupancy of 0.35 (a). An increase of laser power to 30 µJ increases the occupancy of NO to 0.45 (b). A faster timepoint at 100 µs at 30 µJ has a slightly lower occupancy (0.30), suggesting a time dependence of binding (c). At this faster timepoint, a further increase of laser power to 100 µJ again increased the occupancy to 0.45 (d).

IUCrJ
Volume 12| Part 5| September 2025| Pages 582-594
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525006645