view article
Figure 1
Reaction schemes of the oG/oG*–hOgg1 complex. (a) Base excision of oG by hOgg1 followed by DNA cleavage. (b) The reported oG*–hOgg1 system. The bulky O6-NPP group prevents the entry of oG* into the active site of hOgg1. Uncaging of oG by near-UV light triggers accommodation of oG in the active site, followed by a base-excision reaction. For crystallization, the oG*–hOgg1 complex was stabilized by a covalent bond. The cytosine base modified at the N4 position with cystamine (C‡) complementary to oG* is cross-linked with the Cys149 side chain of hOgg1-N149C via a thiol–disulfide exchange reaction.

IUCrJ
Volume 12| Part 5| September 2025| Pages 515-522
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525006062