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Figure 1
Examples of single-particle cryo-EM density maps with missing densities. (a) Single-particle cryo-EM map of human herpesvirus protease (orange) bound to a Fab (teal) with the separate atomic models docked. The Fab and the protease epitope are well resolved. Resolution decreases for the remaining part of the protease with densities disappearing in the distal regions. (b) Single-particle cryo-EM map of integrin αvβ8 (teal) bound to L-TGF-β/GARP (orange/salmon), with the atomic model of αvβ8/L-TGF-β/GARP docked. The resolution of L-TGF-β decreases from the integrin binding site to the distal region. GARP, which is covalently linked to L-TGF-β, is completely invisible in the density map. (c) A top view of the transmembrane domain of the TRPM8 channel from a typical reconstruction without extensive classification with the atomic model docked. The voltage-sensing-like domains (teal) are resolved, but densities for most of the pore domain (orange) are not visible. In all examples, the same density map is shown in high threshold (cyan) and low threshold (transparent gray).

IUCrJ
Volume 13| Part 3| May 2026| Pages 217-220
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526002952