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Figure 1
Crystal structure of abMltB. (a) Overall reaction of lytic transglycosylases. (b) Schematic representation of the overall domain architecture of MltB. (c) Size-exclusion chromatography (SEC) profile of abMltB. SDS–PAGE results used to verify the identity and purity of the protein are displayed adjacent to the major peak, with the loaded fractions highlighted by a black line. M and B indicate marker and before-loaded sample, respectively. (d) The elution volume line fitting in SEC plotted against the size marker and the logarithm of the molecular weight of abMltB. The red point on the fitting line signifies the elution volume of abMltB. The molecular weights of the size markers are indicated along the standard line for reference. (e) Rainbow-colored cartoon model of monomeric abMltB, with a color gradient from blue (N-terminus) to red (C-terminus). Helices and strands are labeled `H' and `S', respectively. (f) Domain organization within the abMltB structure, highlighting the boundary between domains. A putative substrate-binding pocket is marked by a red dotted circle. (g) Surface model of abMltB. (h) Cross-sectional view of the abMltB structure. (i) B-factor distribution visualized using a putty model, with colors ranging from blue (low B factor) to red (high B factor). (j) Multi-angle light scattering (MALS) profile derived from the main size-exclusion chromatography (SEC) peak. The red line indicates the experimental molecular mass analyzed by MALS.

IUCrJ
Volume 13| Part 3| May 2026| Pages 228-234
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526002770