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Figure 1
Comparison of apoferritin single-particle analysis (SPA) with and without the Volta phase plate (VPP). (a, b) Representative micrographs collected without (defocus: 1.31 µm) and with the VPP (defocus: 0.64 µm; phase shift: 70.1°) illustrating the increased low-frequency contrast introduced by the phase plate. (c) Fourier shell correlation (FSC) curves for non-VPP and VPP reconstructions (1.66 Å for non-VPP and 1.90 Å for VPP at the gold-standard FSC = 0.143 criterion). (d) Rosenthal–Henderson plots for non-VPP and VPP datasets (fitted B factors: 67.6 Å2 for non-VPP and 100.8 Å2 for VPP), where KFSC is the inverse of resolution obtained with N particles. (e, f) Apoferritin reconstructions obtained without (1.66 Å) and with the VPP (1.90 Å), shown with docked atomic models highlighting tyrosine and tryptophan side chains.

IUCrJ
Volume 13| Part 3| May 2026| Pages 260-272
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526002575