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Figure 3
Visualization of tomograms acquired with and without a Volta phase plate (VPP): two-protein phantom and near-minimal cells (minicells) Mycoplasma mycoides JCVI-Syn3A. (a, b) 2 nm slabs from the two-protein phantom recorded without VPP [(a), 195 nm thickness measured by AreTomo3] and with VPP [(b), 165 nm thickness]. Magnified insets highlight selected regions reconstructed with weighted back-projection (a1, b1) and after denoising (a2,b2). The green arrow indicates example 80S ribosomes, and the pink arrow marks PP7 virus-like particles. (c, d) Tomograms of bacterial minicells acquired without VPP [(c), 322 nm thickness] and with VPP [(d), 320 nm thickness]. Magnified insets show regions containing 70S ribosomes, presented both before and after denoising.

IUCrJ
Volume 13| Part 3| May 2026| Pages 260-272
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526002575