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Figure 5
Subtomogram average of 80S ribosomes and PP7 virus-like particles (VLPs) from two-protein phantom, and 70S ribosomes from minicells, with and without the Volta phase plate (VPP). (a, b) Subtomogram averaging of the 80S ribosome obtained from non-VPP (a) and VPP (b) tomograms. (c) Fourier shell correlation (FSC) curves for the 80S ribosome, indicating a resolution of 4.8 Å for the non-VPP dataset and 6.8 Å for the VPP dataset using the FSC = 0.143 criterion. (d, e) Subtomogram averaging of PP7 virus-like particles (VLPs) from non-VPP (d) and VPP (e) tomograms. (f) FSC curves for the PP7 VLP reconstructions, indicating a resolution of 4.8 Å from the non-VPP datasets and 7.0 Å from the VPP datasets. (g, h) Subtomogram averaging of 70S ribosomes from non-VPP (g) and VPP (h) tomograms. (i) FSC curves for the 70S ribosome reconstructions, indicating a resolution of 6.6 Å from the non-VPP datasets and 7.0 Å from the VPP datasets. (j–l) Rosenthal–Henderson B-factor plots for non-VPP (blue) and VPP (red) datasets for 80S ribosomes, VLPs and 70S ribosomes, as indicated. Scale bars: 10 nm. Maps are displayed as isosurfaces at 6, 5 and 6 standard deviations from the mean for the 80S ribosome, VLP and 70S ribosome, respectively.

IUCrJ
Volume 13| Part 3| May 2026| Pages 260-272
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526002575