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Figure 1
E. coli chemotaxis signalling as a model system for time-resolved integrative structural biology. (a) Schematic overview of E. coli chemotaxis signalling pathway, highlighting the key components and signalling processes. (b) FRET-based measurement of pathway activity in E. coli. The FRET response, plotted as changes in the YFPCheY to CFPCheZ fluorescence ratio, following sequential additions of serine. Red arrows and numbers indicate key signalling events. (c) Summary of chemotaxis signalling events characterized by time-dependent FRET activity shown in (b). (d) Schematic representation of the mode of action of DMNB-caged serine following light-induced uncaging and its interaction with the chemotaxis system. (e) Minicells as an optimal sample system: they retain the chemotaxis functionality while being smaller, enabling cryoET imaging of chemotaxis signalling complexes. Panel (b) is adapted from Rowe & Parkinson (2023View full citation) under a CC-BY 4.0 licence.

IUCrJ
Volume 13| Part 4| July 2026| Pages 395-408
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526005324