view article
Figure 2
Photophysical characterization of DMNB-caged serine. (a) Photolysis setup featuring a 365 nm LED source and collimating/focusing optics. Samples (5 µl) were deposited on paraffin film and positioned at the location normally occupied by the power sensor, as shown in Supplementary Figure S1. (b) Measured LED output power (mW) as a function of input intensity (%), demonstrating linear behaviour between 0–30% intensity. (c) UV–Vis absorption spectra of the `dark' state of DMNB-caged serine at varying concentrations. Vertical axes represent the maximum wavelength at 350 nm (pink) and the wavelength at which the photophysical characterization was performed (purple). (d) Determination of the molar extinction coefficient (ɛ) at 365 nm from the data in (c) using linear regression. (e) Photolysis kinetics at 365 nm at different LED input intensities (%) within the linear operating regime.

IUCrJ
Volume 13| Part 4| July 2026| Pages 395-408
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526005324