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Figure 3
On-grid UV–Vis spectroscopy. (a) Overview of the experimental setup, highlighting LED light delivery, the microspectrophotometer and the blotting system in blue, yellow and green, respectively. (b) Close-up view of the EM grid positioned above blotters for UV–Vis absorption measurements. (c) UV–Vis absorption kinetic series (0.5–5 s total exposure to light) monitoring DMNB-caged serine release at maximum LED power (192.4 ± 0.3 mW, 100% intensity). The pre-illumination (`dark') on-grid spectrum of the caged serine is shown in dark blue. (d) Photolysis decay kinetics derived from the decrease in absorbance at 365 nm for DMNB-caged serine at maximum LED power.

IUCrJ
Volume 13| Part 4| July 2026| Pages 395-408
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526005324