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Figure 4
Photolysis setup with the PORTO laser and Vitrobot and GC×GC-MS analysis. (a) GC×GC-MS single ion monitoring (SIM) chromatograms showing retention times for DMNB-caged serine and free serine following PORTO laser illumination at 1 µJ of energy. (b) The panel shows the integration of the peak areas for DMNB-caged serine (m/z = 327) and free serine (m/z = 204) before and after laser illumination. The ratios of the integrated peak areas (serine/DMNB-caged serine) are ∼0.7 and ∼1.6 for the samples without and with laser illumination, respectively. (c) Overview of the experimental setup with a Vitrobot, highlighting key components and the laser beam path. (d) Schematic of the experimental apparatus, showing the PORTO laser used for sample irradiation, a fast camera providing high temporal resolution, and the optical assembly comprising a continuous-wave (CW) IR laser, optical fibres, a pair of off-axis parabolic mirrors and a photodiode (PD). The photodiode provides the trigger signal to initiate the measurement sequence, while the Vitrobot controller initiates the motion sequence; associated electronic control units are also indicated.

IUCrJ
Volume 13| Part 4| July 2026| Pages 395-408
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526005324