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Figure 1
High-resolution cryoEM structure of the MATα24βV12 complex. (A) CryoEM Coulomb potential map of the MATα24βV12 complex determined by single-particle cryoEM at 2.6 Å resolution. (B) Composite cryoEM density map showing high-resolution density for the MATα24 core (blue) and high-to-medium resolution density for MATβV1 at the northern end (cornflower blue). (C) Cartoon representation of the complex, highlighting the well defined MATα24 core (blue) and a single, well resolved MATβV1 at the northern end (cornflower blue), alongside partially built MATβV1 subunits at the southern end adopting dual conformations shown in salmon and green. (D) Gaussian-filtered cryoEM map revealing the two conformations of MATβV1 at the southern end. (E) Cartoon model of MATα24 with the northern MATβV1 (cornflower blue) and two MATβV1 subunits (salmon and light green) at the southern end rigid-body fitted into the Gaussian-filtered cryoEM map. (F) Overall view of the MATα2 tetramer (blue) interacting with MATβV1. MATβV1 at the northern end is shown in cornflower blue, while the southern end exhibiting a dual or wobbly conformation is represented in salmon and light green.

IUCrJ
Volume 13| Part 4| July 2026|
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526005075