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Figure 4
High-resolution cryoEM structure of the MATα24βV22 complex. (A) CryoEM Coulomb potential map of the MATα24βV22 complex determined by single-particle cryoEM at 2.6 Å resolution. (B) Composite cryoEM density, displaying high resolution for the MATα24 core (medium slate blue) and lower resolution density for MATβV2 at the northern end (cyan). (C) Cartoon of the enzyme complex showing better-defined MATα24 (medium slate blue) and single MATβV2 (cyan). (D) Gaussian-filtered cryoEM map showing a single conformation of MATβV2 at the northern end and dual or wobbly conformations at the southern end. (E) Cartoon representation of MATα24, with a single MATβV2 at the northern end and two conformations of MATβV2 rigid-body fitted into a Gaussian-filtered cryoEM map. (F) Cartoon representation of the MATα2 tetramer (medium slate blue) interacting with MATβV2. MATβV2 at the northern end is shown in cyan, while the southern end exhibiting a dual conformation is represented in light orange and magenta.

IUCrJ
Volume 13| Part 4| July 2026|
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526005075