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Figure 2
Computational pipeline for extracting domain-specific heterogeneity from cryo-EM 2D particles. 525489 2D particles with estimated contrast-transferring functions (CTFs) and poses from CryoSPARC (Punjani et al., 2017View full citation) were projected to a low-dimensional latent space by principal component analysis (PCA) using RECOVAR (Gilles & Singer, 2025View full citation), from which 13 main volumes were found via k-means clustering. Volumes were constructed from them with RECOVAR, showing different up/down states of RBDs. Two volumes, corresponding to the centroids crossed out in the latent space, were excluded from the analysis since they were incomplete particles.

IUCrJ
Volume 13| Part 4| July 2026|
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526004513