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Figure 1
A drop-on-fixed-target sample-delivery method for tr-MX experiments. (Top) Exploded view of chip mounting for a drop-on-fixed-target experiment. A 6 µm Mylar sheet is applied on the chip mounting base and secured with the outer ring (2 mm high, 52.75 mm diameter spacer). This mitigates sample dehydration from below. The silicon chip sitting on the Mylar sheet is then secured with the open-face chip support. Once the chip is ready it can be easily mounted on the stages via the kinematic mount. (Bottom) Schematic representation of the side view of the chip, highlighting the open and closed side and the distance between the Mylar seal and the wells. The transparent plastic film cover (or cover film) that mitigates dehydration is located at about 0.5 mm from the open-side chip surface and has a 5 × 5 mm hole to allow droplets to pass through. Note that when both sides of the chip are covered with Mylar (e.g. when recording resting-state structures), the top open side of the chip has the Mylar directly covering the wells. Portions of the figure are drawn to scale.

IUCrJ
Volume 13| Part 4| July 2026|
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526003489