forthcoming articles

The viral main protease is indispensable for SARS-CoV-2 replication, and detailed knowledge of its structure and function is crucial to guide structure-aided and computational drug-design efforts. X-ray crystallography is used to reveal the oxidation and reactivity of the protease cysteine residues, providing insights into the enzyme mechanism and for drug design.

Abstract Crystal structure determination of membrane proteins can be challenging. Here, we describe the use of vanadium phasing as an alternative experimental phasing method.

The elusive structure of the mineral kaliophilite was determined by 3D electron diffraction and refined by single-crystal X-ray data. Despite its simple formula, ideally KAlSiO₄, the structure of this mineral remained a mystery for over a century as a result of pseudo-symmetry and twinning, which reduces the coherent crystalline domain size to a few hundreds of nanometres.

Novel approaches for lipid sponge phase crystallization in comparatively large volumes using Hamilton gas tight glass syringes and plastic pipetting tips are described. The selection of distinct co-crystallization conditions allowed to restore in its binding site one of the natural ligands displaced by monoolein.

Structures of monothiol and dithiol glutaredoxin-1 from C. oremlandii are reported. Monothiol glutaredoxin-1 formed domain-swapped structures, one of which was in a hetero-oligomer complex.